Biological test method : reference method for determining acute lethality using threespine stickleback / Method Development and Applications Unit, Science and Technology Branch, Environment and Climate...: En49-7/1-10-2017E-PDF

"Explicit standard or reference methods for measuring the acute lethal toxicity of effluents to marine or estuarine threespine stickleback (Gasterosteus aculeatus) are described in this report. Specific instructions are provided for performing acute lethality tests with effluent samples having a salinity of > 10 g/kg discharging directly to estuarine or marine receiving waters. This second edition report replaces the first edition of Environment Canada’s Biological Test Method EPS 1/RM/10 (EC, 1990a), which was published in July 1990 and amended in March 2000. It supersedes that earlier version, and is to be applied as Environment and Climate Change Canada’s (previously Environment Canada) current reference method for determining the acute lethality of effluents to threespine stickleback”--Abstract, p. ii.

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Publication information
Department/Agency Environmental Science and Technology Centre (Canada). Method Development and Applications Unit.
Canada. Environment and Climate Change Canada.
Title Biological test method : reference method for determining acute lethality using threespine stickleback / Method Development and Applications Unit, Science and Technology Branch, Environment and Climate Change Canada.
Variant title Reference method for determining acute lethality using threespine stickleback
Series title Reference method ; EPS 1/RM/10
Publication type Series - View Master Record
Language [English]
Other language editions [French]
Earlier edition Biological test method - acute lethality test using threespine stickleback (Gasterosteus aculeatus)
Format Electronic
Electronic document
Note(s) Issued also in French under title: Méthode d’essai biologique : méthode de référence pour la détermination de la létalité aiguë à l’aide de l’épinoche à trois épines.
Prepared by: Jennifer Miller … [et al.]. Cf. Acknowledgements.
Includes bibliographical references (p. 27-29).
Issued also in print format.
Publishing information Ottawa : Environment and Climate Change Canada, c2018.
Author / Contributor Miller, Jennifer A. (Jennifer Ann), 1964-
Description xiv, 40 p. : ill. (some col.)
ISBN 9780660243122
Catalogue number
  • En49-7/1-10-2017E-PDF
Edition 2nd ed. Dec. 2017.
Subject terms Water pollution
Water quality
Bioassay
Toxicology
Threespined stickleback
Effluent quality
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