A method to determine cytochrome P4501A activity in wildlife microsomes / S.F. Trudeau and F.J. Maisonneuve.: CW69-5/339E-PDF
"The Biomarker Laboratory (Laboratory Services Section) of the National Wildlife Research Centre (NWRC) has been providing biochemical analyses (biomarkers) in wildlife tissues in support of its National Wildlife Toxicology Program since the early 1990s. Induction of cytochrome P4S01A is a sensitive biomarker of exposure to environmental contaminants such as PARs and HAHs. Measurement of the cytochrome P450-associated enzyme, 7-ethoxyresorufin-O-deethysase (EROD), is the most common method to evaluate the activity of the P4501A system. This report contains a detailed description of a fluorescent spectrophotometric method (MET-lBMK-EROD-01), to measure the product of the EROD reaction (resorufin) and total protein simultaneously, using a plate reader"--Pref.
Permanent link to this Catalogue record:
publications.gc.ca/pub?id=9.858145&sl=0
| Department/Agency | Canada. Environment Canada. Canadian Wildlife Service. Headquarters. National Wildlife Research Centre (Canada) |
|---|---|
| Title | A method to determine cytochrome P4501A activity in wildlife microsomes / S.F. Trudeau and F.J. Maisonneuve. |
| Series title | Technical report series ; no. 339 |
| Publication type | Series - View Master Record |
| Language | [English] |
| Format | Electronic |
| Electronic document | |
| Other formats | Paper-[English] |
| Note(s) | Digitized edition from print [produced by Environment and Climate Change Canada]. Co-published by: National Wildlife Research Centre. Includes bibliographical references. Issued also in print format. Includes preface in French. |
| Publishing information | Hull, Quebec : Canadian Wildlife Service, Headquarters, 2001. |
| Author / Contributor | Trudeau, S. Maisonneuve, F. J. |
| Description | [59] p. |
| Catalogue number |
|
| Subject terms | Wildlife Pollution control Microsomes Cytochrome P-450 |
Request alternate formats
To request an alternate format of a publication, complete the Government of Canada Publications email form. Use the form’s “question or comment” field to specify the requested publication.- Date modified: