00000nam 2200000zi 4500 9.878459 CaOODSP 20240220151437 m o d f cr ||||||||||| 190823t20092009abcd ob f000 0 eng d CaOODSP eng rda CaOODSP eng fre n-cn--- D68-6/279-2009E-PDF Lee, William Edward, author. Quantitative PCR analysis of the decay and recovery of biothreat agents during samplestorage / William E. Lee, Thompson Tang and Douglas E. Bader. Medicine Hat, Alberta : Defence R&D Canada – Suffield, 2009. ©2009 1 online resource (viii, 22 pages, 4 unnumbered pages) : charts. text txt rdacontent computer c rdamedia online resource cr rdacarrier Technical memorandum ; DRDC Suffield TM 2009-279 "December 2009." Includes bibliographical references (pages 21-[22]). "Real-time quantitative polymerase chain reaction (PCR) is widely employed for detection and identification of genetic markers in DNA-containing samples. This report describes methods to use quantitative PCR in conjunction with reaction rate kinetics and thermodynamics to determine recovery of sample material from a commercially available storage medium, FTA® cards. The report shows practitioners of PCR a method to assess analyte recovery, plus the kinetic and thermodynamic parameters associated with sample decay during storage"--Abstract, page i. Includes abstract in French. gccst Biological warfare Tang, Thompson, author. Bader, Douglas E. (Douglas Edward), 1960- author. Defence R&D Canada - Suffield. Technical memorandum (Defence R&D Canada) DRDC Suffield TM 2009-279. (CaOODSP)9.820564 PDF 512 KB https://publications.gc.ca/collections/collection_2019/rddc-drdc/D68-6-279-2009-eng.pdf