Biological test method : test for measuring reproduction of oribatid mites exposed to contaminants in soil / Method Development and Applications Unit, Science and Technology Branch, Environment and Climate...: En83-10/1-61-2020E-PDF

"This document provides detailed procedures, conditions, and guidance on preparing for and conducting a biological test for measuring soil toxicity using an oribatid mite, Oppia nitens. This is a 28-day test for effects on mite reproduction. The method is conducted as a static test using one or more samples of contaminated or potentially contaminated soil, or one or more concentrations of chemical(s) or chemical product(s) spiked in negative control (or other) soil. Water and food (granulated dry yeast) are added to the test vessels during the test. … General or universal conditions and procedures are outlined for test preparation and performance. Additional conditions and procedures specific to the intended use of each test are stipulated. The biological test method described herein is suitable for measuring and assessing the toxicity of samples of field-collected soil, biosolids, sludge, or similar particulate material; or of natural or artificial soil spiked (mixed) in the laboratory with commercial chemical(s) or test substance(s). Instructions and requirements are included for test facilities, sample collection, handling and storing samples, culturing test organisms, preparing soil or spiked-soil mixtures and initiating tests, specific test conditions, appropriate observations and measurements, endpoints and methods of calculation, and the use of positive control replicates or a reference toxicity test"--Abstract, page iii.

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