00000nam 2200000zi 4500 9.921614 CaOODSP 20230501141951 m o d f cr mn||||||||| 230411t20232023qucab ob f000 0 eng d 9780660483368 CaOODSP eng rda CaOODSP eng eng fre n-cn-qu n-cn-nk n-cn-pi y1y2 Fs97-6/3539E-PDF Chevrinais, Marion, author. Targeted detection of European green crab (Carcinus maenas) and Chinese mitten crab (Eriocheir sinensis) using environmental DNA / Marion Chevrinais [and 9 others]. Mont-Joli, QC : Regional Sciences Branch, Quebec region, Benthic and Demersal Sciences Division, Fisheries and Oceans Canada, Maurice Lamontagne Institute, 2023. ©2023 1 online resource (vii, 32 pages) : illustrations (some colour), maps (some colour). text txt rdacontent computer c rdamedia online resource cr rdacarrier Canadian technical report of fisheries and aquatic sciences, 1488-5379 ; 3539 Includes bibliographical references (pages 19-25). "Mitigating the impact of Aquatic Invasive Species (AIS) is critical because of their potential to disturb ecosystems. AIS introduction involves low abundances, which can limit detection with traditional monitoring. Environmental DNA detection (eDNA, or DNA from organisms in the environment) is a relatively sensitive tool for monitoring AIS at low abundances. We present targeted assays for eDNA detection based on quantitative PCR for two AIS, the European green crab (Carcinus maenas) and the Chinese mitten crab (Eriocheir sinensis). In silico validation showed that the C. maenas assay was species-specific whereas the E. sinensis assay also detected two other Eriocheir species (not reported in Canada but potential invaders). Specificity of the 2 assays was assessed in vitro by testing against phylogenetically related species commonly encountered in the Gulf of St. Lawrence, none of which showed DNA amplification. Assay sensitivity was high for both species (limit of detection <2.5 DNA copies/qPCR reaction). Detection of eDNA in situ confirmed findings from traditional monitoring for C. maenas in the Gulf of St. Lawrence. E. sinensis eDNA was detected in the St. Lawrence River but its presence was not confirmed (no active sampling). This study provides targeted eDNA detection protocols with high sensitivity, adapted to Atlantic Canadian waters, and validated to level 4 according to the DFO minimum requirements for eDNA science. These protocols have been implemented for monitoring by the DFO Quebec region since 2019"--Abstract, page vi. Includes abstracts in English and French. Carcinus maenas Detection Saint Lawrence, Gulf of. Carcinus maenas Detection Québec (Province) Saint Lawrence River Estuary. Chinese mitten crab Detection Saint Lawrence River. Chinese mitten crab Detection Québec (Province) Saint Lawrence River Estuary. DNA fingerprinting of animals Saint Lawrence, Gulf of. DNA fingerprinting of animals Québec (Province) Saint Lawrence River Estuary. DNA fingerprinting of animals Saint Lawrence River. Crabe enragé Détection Saint-Laurent, Golfe du. Crabe enragé Détection Québec (Province) Saint-Laurent, Estuaire du. Crabe chinois Détection Saint-Laurent (Fleuve) Crabe chinois Détection Québec (Province) Saint-Laurent, Estuaire du. Animaux Empreintes génétiques Saint-Laurent, Golfe du. Animaux Empreintes génétiques Québec (Province) Saint-Laurent, Estuaire du. Animaux Empreintes génétiques Saint-Laurent (Fleuve) Canada. Department of Fisheries and Oceans, issuing body. Maurice Lamontagne Institute, issuing body. Canadian technical report of fisheries and aquatic sciences ; 3539. (CaOODSP)9.504449 PDF 1.56 MB https://publications.gc.ca/collections/collection_2023/mpo-dfo/Fs97-6-3539-eng.pdf