Larval drift sampling of the Sydenham River fish community, Ontario, in 2017 / by Robin C. Gáspárdy, Jason Barnucz, and D. Andrew R. Drake.: Fs97-13/1477E-PDF

"The Sydenham River in southwestern Ontario supports among the highest richness of freshwater fishes in Canada, including several federally listed species at risk. Assessing the occurrence and spatial distribution of fish larvae can provide insight into the timing and environmental drivers of reproduction for multiple species concurrently. Between April and August of 2017, Fisheries and Oceans Canada (DFO) conducted a multi-week drift sampling program for ichthyoplankton (larval fishes and eggs) in the East Sydenham River. A total of 69 sampling events across 46 field days were conducted with a sampling design that used five 500 μm drift nets (30-minute sets) at three locations (Alvinston, Oil Springs, Florence). In total, 1,670 drift net sets sampled nearly 200,000 m³ of water. Using DNA barcoding to identify individuals to species and metabarcoding to identify species occurrence in bulk samples, this effort resulted in the detection of 48 species representing 16 families as larvae (n=18,418 individuals, 43 species), eggs (n=7,547 individuals, 26 species), and larger (i.e., non-larval) individuals (n=181 individuals, 16 species). Species present in larval drift included Species at Risk Act (SARA)-listed species: Eastern Sand Darter (Ammocrypta pellucida; Threatened), Northern Sunfish (Lepomis peltastes; Special Concern), and Spotted Sucker (Minytrema melanops; Special Concern); and invasive species Round Goby (Neogobius melanostomus) and Common Carp (Cyprinus carpio)"--Abstract, page vi.

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