An environmental DNA-based survey for redside dace (Clinostomus elongatus) in Greater Toronto Area watersheds / by Scott M. Reid, Victoria Kopf and Margaret Boothroyd. : Fs97-4/3120E-PDF

“Redside Dace (Clinostomus elongatus) is a small, colorful cyprinid found in the pools and slow-flowing sections of small Ontario streams. As a result of recent declines and threats to remaining populations, the species has been assessed by COSEWIC as Endangered in Canada. Ongoing inventory efforts are important to delineate areas of protected habitat and monitor trends in the distribution and abundance of Redside Dace populations. Between 2013 and 2015, we collected water samples (five 1-litre samples) from 149 sites in nine Greater Toronto Area watersheds (Bronte Creek, East Morrison Creek, Cold Creek, Kettleby Creek, Lynde Creek, Rainbow Creek, Sharon Creek, Spencer Creek, and Urfe Creek). The presence of Redside Dace at each site was assessed using environmental DNA (eDNA) methods based on species-specific quantitative polymerase chain reaction (qPCR) primers and probe. Only five of the sites sampled for Redside Dace yielded positive eDNA detections. Four positive detections were from Lynde Creek, and one positive detection was from East Morrison Creek. Single-pass backpack electrofishing surveys failed to collect Redside Dace from Lynde and East Morrison creeks. Study results support earlier assessments of declining population status for Redside Dace in these watersheds"--Abstract, p. iv.

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