Review of methods for measuring the toxicity to aquatic organisms of the water accommodated fraction (WAF) and chemically-enhanced water accommodated fraction (CEWAF) of petroleum / Julie Adams ... [et... : Fs70-5/2017-064E-PDF

“The shipment of diluted bitumen (dilbit) to Canadian refineries and ports is increasing with the expansion of its production in northern Alberta. Dilbit has unique physical and chemical properties relative to conventional crude oils, including rapid weathering and greater adhesion and viscosity that affect its fate, behaviour and effects in marine and freshwater ecosystems. The Chemical Response to Oil Spills: Ecological Effects Research Forum (CROSERF) recommended standard protocols to prepare Water Accommodated Fractions (WAFs) and Chemically-Enhanced Water Accommodated Fractions (CEWAFs) of crude oil for toxicity tests. This review updates the CROSERF protocols with recommendations to overcome specific issues with dilbit. The advantages and disadvantages of various methods are reviewed and a proposed framework is provided on experimental designs, physical and chemical characterization of test oils, preparation of physically- and chemically-dispersed oil, characterizing test solutions, and toxicity testing. Research needs are presented to improve experimental designs, the preparation and toxicity testing of oil solutions, the chemical characterization of test oil and oil solutions, and test requirements that recognize the unique characteristics of dilbit and other crude and refined oils"--Abstract, p. ix.

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