00000nam 2200000za 4500 9.858145 CaOODSP 20241203113643 cr ||||||||||| 180626s2001 quc o f00| 0 eng d CaOODSP eng eng fre n-cn--- CW69-5/339E-PDF Trudeau, S. A method to determine cytochrome P4501A activity in wildlife microsomes [electronic resource] / S.F. Trudeau and F.J. Maisonneuve. Hull, Quebec : Canadian Wildlife Service, Headquarters, 2001. [59] p. Technical report series ; no. 339 Digitized edition from print [produced by Environment and Climate Change Canada]. Co-published by: National Wildlife Research Centre. Includes bibliographical references. "The Biomarker Laboratory (Laboratory Services Section) of the National Wildlife Research Centre (NWRC) has been providing biochemical analyses (biomarkers) in wildlife tissues in support of its National Wildlife Toxicology Program since the early 1990s. Induction of cytochrome P4S01A is a sensitive biomarker of exposure to environmental contaminants such as PARs and HAHs. Measurement of the cytochrome P450-associated enzyme, 7-ethoxyresorufin-O-deethysase (EROD), is the most common method to evaluate the activity of the P4501A system. This report contains a detailed description of a fluorescent spectrophotometric method (MET-lBMK-EROD-01), to measure the product of the EROD reaction (resorufin) and total protein simultaneously, using a plate reader"--Pref. Issued also in print format. Includes preface in French. gccst Wildlife gccst Pollution control Microsomes Cytochrome P-450 Maisonneuve, F. J. Canada. Environment Canada. Canadian Wildlife Service. Headquarters. National Wildlife Research Centre (Canada) A method to determine cytochrome P4501A activity in wildlife microsomes / (CaOODSP)9.614888 Technical report series (Canadian Wildlife Service) no. 339. (CaOODSP)9.504438 PDF 1.73 MB https://publications.gc.ca/collections/collection_2018/eccc/cw69-5/CW69-5-339-eng.pdf