00000nam 2200000zi 4500 9.873306 CaOODSP 20241203113648 m o d f cr bn||||||||| 190521e198904##oncd ob f000 0 eng d CaOODSP eng rda CaOODSP eng fre n-cn-on En13-5/89-11E-PDF Burnison, B. K., author. Purification of DNA for bacterial productivity estimates / B. Kent Burnison and Donna J. Nuttley. Burlington, Ontario : Lakes Research Branch, National Water Research Institute, Canada Centre for Inland Waters, April 1989. 1 online resource (17 pages) : chart. text txt rdacontent computer c rdamedia online resource cr rdacarrier NWRI contribution ; no. 89-11 Digitized edition from print [produced by Environment and Climate Change Canada]. Includes bibliographical references (pages 10-14). "[Methyl-3H] thymidine labelled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein using hydroxylapatite chromatography. The procedure was validated by monitoring increases in E; coli cell count, absorbance at 550 nm, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolyses or volatile organic solvents"--Abstract. Includes management perspective and abstract in French. gccst Aquatic ecosystems gccst Bacteria Nuttley, Donna J., author. Canada. Environment Canada. National Water Research Institute (Canada). Lakes Research Branch. Canada Centre for Inland Waters. NWRI contribution ; no. 89-11. (CaOODSP)9.844121 PDF 1.25 MB https://publications.gc.ca/collections/collection_2019/eccc/en13-5/En13-5-89-11-eng.pdf