000	02109cam  2200397zi 4500
001	9.873306
003	CaOODSP
005	20221107163857
006	m     o  d f
007	cr bn|||||||||
008	190521e198904##oncd    ob   f000 0 eng d
040	|aCaOODSP|beng|erda|cCaOODSP
041	|aeng|bfre
043	|an-cn-on
086	1 |aEn13-5/89-11E-PDF
100	1 |aBurnison, B. K., |eauthor.
245	10|aPurification of DNA for bacterial productivity estimates / |cB. Kent Burnison and Donna J. Nuttley.
264	1|aBurlington, Ontario : |bLakes Research Branch, National Water Research Institute, Canada Centre for Inland Waters, |cApril 1989.
300	|a1 online resource (17 pages) : |bchart.
336	|atext|btxt|2rdacontent
337	|acomputer|bc|2rdamedia
338	|aonline resource|bcr|2rdacarrier
490	1 |aNWRI contribution ; |vno. 89-11
500	|aDigitized edition from print [produced by Environment and Climate Change Canada].
504	|aIncludes bibliographical references (pages 10-14).
520	3 |a"[Methyl-3H] thymidine labelled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein using hydroxylapatite chromatography. The procedure was validated by monitoring increases in E; coli cell count, absorbance at 550 nm, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolyses or volatile organic solvents"--Abstract.
546	|aIncludes management perspective and abstract in French.
692	07|2gccst|aAquatic ecosystems
692	07|2gccst|aBacteria
700	1 |aNuttley, Donna J., |eauthor.
710	1 |aCanada. |bEnvironment Canada.
710	2 |aNational Water Research Institute (Canada). |bLakes Research Branch.
710	2 |aCanada Centre for Inland Waters.
830	#0|aNWRI contribution ;|vno. 89-11.|w(CaOODSP)9.844121
856	40|qPDF|s1.25 MB|uhttps://publications.gc.ca/collections/collection_2019/eccc/en13-5/En13-5-89-11-eng.pdf