00000nam 2200000zi 4500 9.873823 CaOODSP 20241203113649 m o d f cr |n||||||||| 190529s1989 oncd #ot f000 0 eng d CaOODSP eng rda CaOODSP eng fre n-cn--- En13-5/89-130E-PDF Sherry, J. P., author. Dimethyl sulfoxide as solubilization agent in the radioimmunoassay for the detection of polychlorinated dibenzo-p-dioxins / by J.P. Sherry [and three others]. Burlington, Ontario : Research and Applications Branch, National Water Research Institute, [1989] 1 online resource (ii, ii, 16 pages) : figures. text txt rdacontent computer c rdamedia online resource cr rdacarrier NWRI contribution ; no. 89-130 Digitized edition from print [produced by Environment and Climate Change Canada]. Includes bibliographical references. "Immunoassays are potentially valuable tools for use in screening environmental samples for a broad range of contaminants, such as polychlorinated dibenzo-p-dioxins (PCDDs). The performance of the RIA for PCDDs was characterized using 4 solubilization systems: Cutscum, Triton, horse serum, and dimethylsulfoxide (DMSO). The DMSO based assay appeared to perform best at low PCDD levels. The effects of assay incubation time and hapten storage conditions on the DMSO based assay were assessed. The separation of bound from unbound radioactivity was accelerated without adversely affecting assay performance. Further assay development through the use of an improved hapten is considered"--Abstract. Includes abstract in French. gccst Water pollution Canada. Environment Canada. National Water Research Institute (Canada). Research and Applications Branch. NWRI contribution ; no. 89-130. (CaOODSP)9.844121 PDF 1.24 MB https://publications.gc.ca/collections/collection_2019/eccc/en13-5/En13-5-89-130-eng.pdf