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008190823t20092009abcd    ob   f000 0 eng d
040 |aCaOODSP|beng|erda|cCaOODSP
041 |aeng|bfre
043 |an-cn---
0861 |aD68-6/279-2009E-PDF
1001 |aLee, William Edward, |eauthor.
24510|aQuantitative PCR analysis of the decay and recovery of biothreat agents during samplestorage / |cWilliam E. Lee, Thompson Tang and Douglas E. Bader.
264 1|aMedicine Hat, Alberta : |bDefence R&D Canada – Suffield, |c2009.
264 4|c©2009
300 |a1 online resource (viii, 22 pages, 4 unnumbered pages) : |bcharts.
336 |atext|btxt|2rdacontent
337 |acomputer|bc|2rdamedia
338 |aonline resource|bcr|2rdacarrier
4901 |aTechnical memorandum ; |vDRDC Suffield TM 2009-279
500 |a"December 2009."
504 |aIncludes bibliographical references (pages 21-[22]).
5203 |a"Real-time quantitative polymerase chain reaction (PCR) is widely employed for detection and identification of genetic markers in DNA-containing samples. This report describes methods to use quantitative PCR in conjunction with reaction rate kinetics and thermodynamics to determine recovery of sample material from a commercially available storage medium, FTA® cards. The report shows practitioners of PCR a method to assess analyte recovery, plus the kinetic and thermodynamic parameters associated with sample decay during storage"--Abstract, page i.
546 |aIncludes abstract in French.
69207|2gccst|aBiological warfare
7001 |aTang, Thompson, |eauthor.
7001 |aBader, Douglas E. |q(Douglas Edward), |d1960- |eauthor.
7101 |aDefence R&D Canada - Suffield.
830#0|aTechnical memorandum (Defence R&D Canada)|vDRDC Suffield TM 2009-279.|w(CaOODSP)9.820564
85640|qPDF|s512 KB|uhttps://publications.gc.ca/collections/collection_2019/rddc-drdc/D68-6-279-2009-eng.pdf