| 000 | 00000nam 2200000zi 4500 |
| 001 | 9.878459 |
| 003 | CaOODSP |
| 005 | 20240220151437 |
| 006 | m o d f |
| 007 | cr ||||||||||| |
| 008 | 190823t20092009abcd ob f000 0 eng d |
| 040 | |aCaOODSP|beng|erda|cCaOODSP |
| 041 | |aeng|bfre |
| 043 | |an-cn--- |
| 086 | 1 |aD68-6/279-2009E-PDF |
| 100 | 1 |aLee, William Edward, |eauthor. |
| 245 | 10|aQuantitative PCR analysis of the decay and recovery of biothreat agents during samplestorage / |cWilliam E. Lee, Thompson Tang and Douglas E. Bader. |
| 264 | 1|aMedicine Hat, Alberta : |bDefence R&D Canada – Suffield, |c2009. |
| 264 | 4|c©2009 |
| 300 | |a1 online resource (viii, 22 pages, 4 unnumbered pages) : |bcharts. |
| 336 | |atext|btxt|2rdacontent |
| 337 | |acomputer|bc|2rdamedia |
| 338 | |aonline resource|bcr|2rdacarrier |
| 490 | 1 |aTechnical memorandum ; |vDRDC Suffield TM 2009-279 |
| 500 | |a"December 2009." |
| 504 | |aIncludes bibliographical references (pages 21-[22]). |
| 520 | 3 |a"Real-time quantitative polymerase chain reaction (PCR) is widely employed for detection and identification of genetic markers in DNA-containing samples. This report describes methods to use quantitative PCR in conjunction with reaction rate kinetics and thermodynamics to determine recovery of sample material from a commercially available storage medium, FTA® cards. The report shows practitioners of PCR a method to assess analyte recovery, plus the kinetic and thermodynamic parameters associated with sample decay during storage"--Abstract, page i. |
| 546 | |aIncludes abstract in French. |
| 692 | 07|2gccst|aBiological warfare |
| 700 | 1 |aTang, Thompson, |eauthor. |
| 700 | 1 |aBader, Douglas E. |q(Douglas Edward), |d1960- |eauthor. |
| 710 | 1 |aDefence R&D Canada - Suffield. |
| 830 | #0|aTechnical memorandum (Defence R&D Canada)|vDRDC Suffield TM 2009-279.|w(CaOODSP)9.820564 |
| 856 | 40|qPDF|s512 KB|uhttps://publications.gc.ca/collections/collection_2019/rddc-drdc/D68-6-279-2009-eng.pdf |