Application of germination inhibitors in organic solvents to conifer seeds / A. Groot. : Fo46-14/371-1985E-PDF

"Black spruce (Picea mariana [Mill.] B.S.P.) seeds were immersed in dichloromethane and acetone for 20 hours at 20°C with no ill effects on germination, but shorter immersion periods and lower temperatures were necessary to avoid affecting jack pine (Pinus banksiana Lamb.) germination. Dichloromethane did not appear to penetrate to the megagametophyte of black spruce seed. Abscisic acid, applied in these solvents, inhibited the germination of black spruce seeds effectively without reducing viability. This inhibition was partially reversed by cold stratification and by overwintering, but not by leaching. The germination of jack pine seeds was also inhibited by abscisic acid applied in acetone or in water, but to a lesser degree than with black spruce seeds. Stirring the solution during jack pine seed immersion increased the response to abscisic acid. Coumarin had no effect on the germination of either species"--Abstract.

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