Biological test method : tests for measuring avoidance behaviour or reproduction of earthworms (Eisenia andrei or Dendrodrilus rubidus) exposed to contaminants in soil / Method Development and Applications... : En14-497/2022E-PDF

"Revised methods now recommended by Environment and Climate Change Canada for conducting biological test methods for measuring soil toxicity using earthworms (Eisenia andrei and Dendrodrilus rubidus) are described in this report. This revised version of Report EPS 1/RM/43 includes numerous updates such as: guidance for the collection, handling and testing of soils; the removal of two test species (Eisenia fetida and Lumbricus terrestris) and the addition of another test species (Dendrodrilus rubidus) specifically for testing soils from the boreal or taiga ecozones; revised test designs; and updated guidance for culturing and testing, testing with a reference toxicant, and for the statistical analysis of data. In addition, due to lack of demand and lack of sensitivity relative to the reproduction test, the 14-day acute lethality test has been removed as a test option herein. This revised report supersedes the first edition of this document published as Report EPS 1/RM/43 in June 2004 and revised in June 2007. This test method document provides detailed procedures, conditions, and guidance for preparing for and conducting each of two discrete biological test methods for measuring soil toxicity using earthworms (Eisenia andrei or Dendrodrilus rubidus). The test methods described herein are as follows: i) a 56-day test for effects on reproduction of adult earthworms exposed to one or more samples or concentrations of contaminated or potentially contaminated soil; and ii) a 48-hour test for avoidance of contaminated soil by adult earthworms"--Abstract, page iii.

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