00000cam 2200000zi 4500 9.959271 CaOODSP 20260206142924 m o d f cr mn |||||||| 260126t20262026bccabd ob f000 0 eng d 9780660981093 CaOODSP eng rda CaOODSP eng eng fre n-cn-bc Fs97-6/3753E-PDF Abbott, Cathryn, author. National Aquatic Animal Health Laboratory System report on the development and validation of a qPCR assay for detection of Mikrocytos mackini / by Cathryn Abbott, Mark Polinski, Geoff Lowe, Scott Gilmore, Gary Meyer, Eliah Kim and Anne-Marie Flores. Nanaimo, BC : Fisheries and Oceans Canada, Science Branch, Pacific Region, Pacific Biological Station, 2026. ©2026 1 online resource (ix, 22 pages) : illustrations, maps, graphs. text txt rdacontent computer c rdamedia online resource cr rdacarrier Canadian technical report of fisheries and aquatic sciences, 1488-5379 ; 3753 Includes bibliographical references (pages 20-22). "Mikrocytos mackini is a protistan parasite that causes Denman Island Disease, or 'mikrocytosis', in Pacific oysters (Crassostrea gigas), which is a federally regulated disease in Canada. High sensitivity detection of M. mackini has several applications, including disease surveillance and import/export testing, hence we developed and diagnostically validated a specific TaqMan qPCR assay"--Abstract, page viii. Includes abstract in French. Polymerase chain reaction. Intracellular pathogens Detection British Columbia Pacific Coast. Oysters Diseases British Columbia Pacific Coast. Réaction en chaîne de la polymérase. Micro-organismes pathogènes intracellulaires Détection Colombie-Britannique Pacifique, Côte du. Huîtres Maladies Colombie-Britannique Pacifique, Côte du. Pacific Biological Station (1972- ), issuing body. Canadian technical report of fisheries and aquatic sciences, 1488-5379 ; 3753. (CaOODSP)9.504449 PDF 1.10 MB https://publications.gc.ca/collections/collection_2026/mpo-dfo/fs97-6/Fs97-6-3753-eng.pdf