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Purification of DNA for bacterial productivity estimates / B. Kent Burnison and Donna J. Nuttley.: En13-5/89-11E-PDF

"[Methyl-3H] thymidine labelled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein using hydroxylapatite chromatography. The procedure was validated by monitoring increases in E; coli cell count, absorbance at 550 nm, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolyses or volatile organic solvents"--Abstract.

Permanent link to this Catalogue record:
publications.gc.ca/pub?id=9.873306&sl=0

Publication information
Department/Agency
  • Canada. Environment Canada.
  • National Water Research Institute (Canada). Lakes Research Branch.
  • Canada Centre for Inland Waters.
TitlePurification of DNA for bacterial productivity estimates / B. Kent Burnison and Donna J. Nuttley.
Series title
  • NWRI contribution ; no. 89-11
Publication typeMonograph - View Master Record
Language[English]
FormatDigital text
Electronic document
Note(s)
  • Digitized edition from print [produced by Environment and Climate Change Canada].
  • Includes bibliographical references (pages 10-14).
  • Includes management perspective and abstract in French.
Publishing information
  • Burlington, Ontario : Lakes Research Branch, National Water Research Institute, Canada Centre for Inland Waters, April 1989.
Author / Contributor
  • Burnison, B. K., author.
  • Nuttley, Donna J., author.
Description1 online resource (17 pages) : chart.
Catalogue number
  • En13-5/89-11E-PDF
Subject terms
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