Purification of DNA for bacterial productivity estimates / B. Kent Burnison and Donna J. Nuttley. : En13-5/89-11E-PDF
"[Methyl-3H] thymidine labelled DNA from natural populations of aquatic bacteria was completely separated from RNA and protein using hydroxylapatite chromatography. The procedure was validated by monitoring increases in E; coli cell count, absorbance at 550 nm, DNA concentration, and thymidine incorporation into DNA isolated by the proposed technique. The procedure can be used in the field and does not rely on the use of acid-base hydrolyses or volatile organic solvents"--Abstract.
Lien permanent pour cette publication :
publications.gc.ca/pub?id=9.873306&sl=1
| Ministère/Organisme | Canada. Environment Canada. National Water Research Institute (Canada). Lakes Research Branch. Canada Centre for Inland Waters. |
|---|---|
| Titre | Purification of DNA for bacterial productivity estimates / B. Kent Burnison and Donna J. Nuttley. |
| Titre de la série | NWRI contribution ; no. 89-11 |
| Type de publication | Série - Voir l'enregistrement principal |
| Langue | [Anglais] |
| Format | Électronique |
| Document électronique | |
| Note(s) | Digitized edition from print [produced by Environment and Climate Change Canada]. Includes bibliographical references (pages 10-14). Includes management perspective and abstract in French. |
| Information sur la publication | Burlington, Ontario : Lakes Research Branch, National Water Research Institute, Canada Centre for Inland Waters, April 1989. |
| Auteur / Contributeur | Burnison, B. K., author. Nuttley, Donna J., author. |
| Description | 1 online resource (17 pages) : chart. |
| Numéro de catalogue |
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| Descripteurs | Aquatic ecosystems Bacteria |
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