Quantitative PCR analysis of the decay and recovery of biothreat agents during samplestorage / William E. Lee, Thompson Tang and Douglas E. Bader.: D68-6/279-2009E-PDF

"Real-time quantitative polymerase chain reaction (PCR) is widely employed for detection and identification of genetic markers in DNA-containing samples. This report describes methods to use quantitative PCR in conjunction with reaction rate kinetics and thermodynamics to determine recovery of sample material from a commercially available storage medium, FTA® cards. The report shows practitioners of PCR a method to assess analyte recovery, plus the kinetic and thermodynamic parameters associated with sample decay during storage"--Abstract, page i.

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Publication information
Department/Agency Defence R&D Canada - Suffield.
Title Quantitative PCR analysis of the decay and recovery of biothreat agents during samplestorage / William E. Lee, Thompson Tang and Douglas E. Bader.
Series title Technical memorandum ; DRDC Suffield TM 2009-279
Publication type Series - View Master Record
Language [English]
Format Electronic
Electronic document
Note(s) "December 2009."
Includes bibliographical references (pages 21-[22]).
Includes abstract in French.
Publishing information Medicine Hat, Alberta : Defence R&D Canada – Suffield, 2009.
©2009
Author / Contributor Lee, William Edward, author.
Tang, Thompson, author.
Bader, Douglas E. (Douglas Edward), 1960- author.
Description 1 online resource (viii, 22 pages, 4 unnumbered pages) : charts.
Catalogue number
  • D68-6/279-2009E-PDF
Subject terms Biological warfare
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