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Dimethyl sulfoxide as solubilization agent in the radioimmunoassay for the detection of polychlorinated dibenzo-p-dioxins / by J.P. Sherry [and three others]. : En13-5/89-130E-PDF

"Immunoassays are potentially valuable tools for use in screening environmental samples for a broad range of contaminants, such as polychlorinated dibenzo-p-dioxins (PCDDs). The performance of the RIA for PCDDs was characterized using 4 solubilization systems: Cutscum, Triton, horse serum, and dimethylsulfoxide (DMSO). The DMSO based assay appeared to perform best at low PCDD levels. The effects of assay incubation time and hapten storage conditions on the DMSO based assay were assessed. The separation of bound from unbound radioactivity was accelerated without adversely affecting assay performance. Further assay development through the use of an improved hapten is considered"--Abstract.

Lien permanent pour cette publication :
publications.gc.ca/pub?id=9.873823&sl=1

Renseignements sur la publication
Ministère/Organisme
  • Canada. Environment Canada.
  • National Water Research Institute (Canada). Research and Applications Branch.
TitreDimethyl sulfoxide as solubilization agent in the radioimmunoassay for the detection of polychlorinated dibenzo-p-dioxins / by J.P. Sherry [and three others].
Titre de la série
  • NWRI contribution ; no. 89-130
Type de publicationMonographie - Voir l'enregistrement principal
Langue[Anglais]
FormatTexte numérique
Document électronique
Note(s)
  • Digitized edition from print [produced by Environment and Climate Change Canada].
  • Includes bibliographical references.
  • Includes abstract in French.
Information sur la publication
  • Burlington, Ontario : Research and Applications Branch, National Water Research Institute, [1989]
Auteur / Contributeur
  • Sherry, J. P., author.
Description1 online resource (ii, ii, 16 pages) : figures.
Numéro de catalogue
  • En13-5/89-130E-PDF
Descripteurs
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